The cees dekker Diaries

The cees dekker Diaries

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Nanotechnology offers a range of options to lead to biology. Below I'll existing illustrations where by nanofabrication – a chance to make confined structures with dimensions at will – is utilised to examine the biophysics of solitary molecules and cells. I will current some examples from our lab:

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Unfortunately, For a long time following Iijima’s report, the manufacture of clean nanotubes was cumbersome. In 1996, on the other hand, the group of Richard Smalley at Rice University managed to generate single-walled carbon nanotubes at significant yield. Shortly just after, I contacted Smalley, and we decided to be part of forces to evaluate the transport by way of a one carbon nanotube.

2012, discovery that nucleoid occlusion underlies the accuracy of bacterial mobile division; and very first at any time examine with the dynamics DNA supercoils and the invention of supercoil hopping

Experimental phase diagram of negatively supercoiled DNA measured by magnetic tweezers and fluorescence,

Considering that 2000 he moved to solitary-molecule biophysics and nanobiology, with study from DNA supercoiling experiments of nucleosomes and DNA repair service proteins to DNA translocation by way of nanopores. Not too long ago his study has centered on finding out cell division with microbes on chip, even though his greatest desire is from the path of realizing artificial cells.

Atomic force microscopy exhibits that vaccinia topoisomerase IB generates filaments on DNA in the cooperative manner

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But carbon nanotubes have remained a exploration matter of significant curiosity to numerous. Indeed, the outlook on using carbon nanotubes to build simple products, in parts for example radio-frequency electronics, digital electronics and flexible electronics, continues to be shiny.

FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in bacterial cell division

‘That considerations the artificial cell, as Element of the Dutch BaSyC initiative. Craig Venter looked top rated-down in those days and discovered about five hundred genes which might be minimally demanded for any operating mobile. We cees dekker want to search base-up: how can we move from a several molecules to a complete biological technique?

Electrical transport in monolayers of phthalocyanine molecular wires and AFM imaging of an individual wire bridging two electrodes

 Last but not least, I'll briefly sketch several of our Strategies to take a look at the building of artificial cells, specifically our initial actions to establish synthetic cell division.